Data · dataset · 2021
Replication Data for: Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains
Listed in International Potato Center
Potato virus Y (PVY) and Phytophthora infestans (Mont.) de Bary that causes potato late blight (LB), pose serious constraints to cultivated potatoes due to significant yield reduction, and phenotyping for resistance remains challenging.
Description
Breeding operations for vegetatively propagated crops can lead to genotype mislabeling that, in turn, reduces genetic gains. Low-density and low-cost molecular marker assessment for phenotype prediction and quality control is a viable option for breeding programs.
Here, we report on the development of kompetitive allele specific PCR (KASP) markers for LB and PVY resistance, and for routine quality control assessment of different breeding populations. Two KASP markers for LB resistance and two for PVY Ryadg were validated with an estimated assay power that ranged between 0.65 and 0.88. The developed QC KASP markers demonstrated the capability of discriminating tetraploid calls in breeding materials, including fullsibs and half-sibs.
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Routine implementation of the developed markers in a breeding program would assist with better allocation of resources and enable precise characterization of breeding material, thereby leading to increased genetic gains.
Links
Where it is published
- Dataverse dataset page data.cipotato.org/dataset.xhtml?persistentId=doi%3A10.21223%2FU9H0X0 ↗
landing page · from data cipotato org
- DOI doi.org/10.21223/u9h0x0 ↗
DOI / persistent id · from data cipotato org
Catalogue records · 1
- Dataverse API data.cipotato.org/api/datasets/:persistentId/?persistentId=doi%3A10.21223%2FU9H0… ↗
metadata API · from data cipotato org
Topics
- Stated by source
- Agricultural Sciences
- From keywords
- Life Sciences
- Inferred from text
- Agricultural biotechnology 69%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| International Potato Center | doi:10.21223/U9H0X0 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3001 | enrichment · data cipotato org | taxonomy-embedding@1.0.0 | title+keywords+description (69%) |
| concepts[field].dataverse_subject:agricultural-sciences | source · data cipotato org | connector:data_cipotato_org@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · data cipotato org | connector:data_cipotato_org@1.0.0 | /subjects |
| created_date | source · data cipotato org | connector:data_cipotato_org@1.0.0 | |
| description | source · data cipotato org | connector:data_cipotato_org@1.0.0 | /description |
| publication_date | source · data cipotato org | connector:data_cipotato_org@1.0.0 | |
| title | source · data cipotato org | connector:data_cipotato_org@1.0.0 | /name |
| updated_date | source · data cipotato org | connector:data_cipotato_org@1.0.0 | |
| version_label | source · data cipotato org | connector:data_cipotato_org@1.0.0 |