Data · dataset · 2026
The concatenated nuclear gene and plastome datasets of the Aralia-Panax complex, and the Aralia-Panax complex occurrences
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Total genomic DNA extraction and quality control were reported in detail by Liu et al. (2023) .
Description
The baits for the 936 genes used for target enrichment and sequencing have been detailed by Gallego‐Narbón et al. (2022) . We first trimmed the raw reads using Trimmomatic v.0.39 (Bolger et al., 2014) with a quality cutoff of 20. We removed bases at the beginning and the end of each read with a Phred quality score below 3, and discarded any reads trimmed to under 36 nucleotides.
We then used the HybPiper pipeline v.1.3.1 (Johnson et al., 2016) to extract genes for downstream phylogenetic and biogeographic analyses. This suite of programs uses BWA 0.7.17 (Li and Durbin, 2009) to assign reads to target genes, and reads are then assembled separately for each target using SPAdes 3.12.0 (Bankevich et al., 2012). Potential paralogs were detected and removed from the sequence dataset using the “paralog investigator” tool included in HybPiper.
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This resulted in 624 loci being removed from the final dataset and subsequent analyses. Therefore, the final dataset included 312 orthologs.For plastid genome assembly, we firstly extracted coding genes and intergenic spacers from the reference genome of Panax bipinnatifidus (GenBank accession NC043952). Consistent with the nuclear Hyb-Seq analytical pipeline, we mapped trimmed clean reads to the plastid loci using BWA, and reads corresponding to each plastid locus were assembled separately with SPAdes.
The plastome matrix had an aligned length of 128,839 bp.The Aralia-Panax complex occurrences were obtained from the Global Biodiversity Information Facility (doi.org/10.15468/dl.n25jar; doi.org/10.15468/dl.wuuwj3). Only specimen records were retained, records with missing or invalid geographic coordinates, obvious georeferencing errors, and duplicated localities were subsequently removed. To reduce spatial sampling bias and spatial autocorrelation, occurrence records were spatially rarefied by retaining a single record within a minimum distance of 10 km.
After spatial rarefaction, a final geo-referenced dataset with 6259 occurrence points was compiled for modelling the distribution of the Aralia-Panax complex.
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Where it is published
- DOI doi.org/10.57760/sciencedb.0110m ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Physics · Social Science
- Inferred from text
- Genetics 72% · Sequencing 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.0110m | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (72%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:physics | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |