<p>Dengue infected patients display IgG-SC of higher affinity for DENV1 sE-dimers than DENV2 sE dimers irrespective of the infecting serotype.</p>

<p><b>(A-B)</b> DropMap-measured affinity (KD) of IgG-SC from indicated <b>(A)</b> subclinical or <b>(B)</b> hospitalized patients infected with DENV1. <b>(C-D)</b> DropMap-measured affinity (KD) of IgG-SC from individual <b>(C)</b> subclinical or <b>(D)</b> hospitalized patients infected with DENV2. <b>(A-D)</b> Data are divided by categories of IgG-SC that bind one sE-dimer or both sE-dimers, as indicated in the legend below the graphs.

Horizontal bars indicate median and interquartile range. Each dot represents the KD value of one IgG-SC recognizing at least one sE-dimer. p values were calculated using two-by-two contrast comparisons after linear modelling. ns, not significant; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. The dotted line indicates the 30nM affinity limit that discriminates E protein binders from non-binders.