Data · dataset
[16S rRNA gene from RNA] - 16S rRNA gene (from RNA) from samples collected on cruise Chikyu-331 in the Okinawa Trough, Japan from September to October 2010 (An In-Depth analysis of the subvent biosphere within Okinawa Backarc Basin (IODP 331, Iheya North Hydrothermal Field) sediments)
Listed in Biological and Chemical Oceanography Data Management Office (BCO-DMO) ERDDAP
RNA extractions were attempted from sediments at Sites C0014 and C0017 to selectively investigate the active microbial populations at these sites.
Description
This study was conducted to supplement the gene-based and metagenomic studies. However, the results from sequenced reverse transcribe 16S rRNA demonstrate the sensitivity of extraction and amplification to external nucleic acids and attest to the challenges in working with low biomass environmental samples.
Inconsistencies between primersets and replicates make conclusions about the results of this RNA study extremely tenuous.
Links
Where it is published
- ERDDAP dataset info erddap.bco-dmo.org/erddap/info/bcodmo_dataset_781114/index.html ↗
landing page · from erddap bco dmo org
Documentation and papers
- Dataset documentation bco-dmo.org/dataset/781114 ↗
documentation · from erddap bco dmo org
Catalogue records · 1
- ERDDAP tabledap access form erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_781114.html ↗
metadata API · from erddap bco dmo org
Topics
- From keywords
- Ocean & Atmospheric Science
Provenance · 1 source records, 5 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Biological and Chemical Oceanography Data Management Office (BCO-DMO) ERDDAP | bcodmo_dataset_781114 | 6 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · erddap bco dmo org | connector:erddap_bco_dmo_org@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · erddap bco dmo org | connector:erddap_bco_dmo_org@1.0.0 | |
| description | source · erddap bco dmo org | connector:erddap_bco_dmo_org@1.0.0 | /summary |
| spatial | source · erddap bco dmo org | connector:erddap_bco_dmo_org@1.0.0 | |
| title | source · erddap bco dmo org | connector:erddap_bco_dmo_org@1.0.0 | /title |