Archive · dataset · 2021
Benthic bacterial communities in sandy surface sediments (Helgoland Roads, North Sea)
Listed in PANGAEA
Coastal sands are biocatalytic filters for dissolved and particulate organic matter of marine and terrestrial origin.
Description
In this study, we accessed the variability of benthic bacterial communities over two annual cycles at Helgoland Roads (North Sea, German Bight) sediments, where primary production does not occur during winter. Sediment cores were obtained by scientific divers.
Benthic community structure in surface sediments (0-2 cm depth) was studied by 16S rRNA-based taxonomy (16S rRNA gene sequencing using primers S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21, Illumina sequencing platform HiSeq2500, 2x250 bases, paired-end). Total organic carbon content was determined using 25-50 mg of freeze-dried sediments. To determine organic carbon, inorganic carbon was removed with 1 M HCl before analysis.
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Samples were analyzed in an Euro EA-CNS elemental analyser (HEKAtech, column temperature 75°C; carrier gas helium at 80 ml min-1, reactor temperature 1000°C with oxygen flow 10 ml min-1, oxidation time 7.9 sec) with thermal conductivity detection. Calibration was done with sulfanilamide (0.2 to 2 mg) standards.
Links
Where it is published
- DOI doi.org/10.1594/pangaea.930442 ↗
DOI / persistent id · from PANGAEA
Catalogue records · 1
- OAI-PMH record ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=oai_dc&identifier=o… ↗
metadata API · from PANGAEA
Topics
- From keywords
- Earth & Environmental Science
- Inferred from text
- Pollution and contamination 72% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| PANGAEA | oai:pangaea.de:doi:10.1594/PANGAEA.930442 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · PANGAEA | connector:pangaea@1.0.0 | |
| concepts[field].anzsrc:group:4105 | enrichment · PANGAEA | taxonomy-embedding@1.0.0 | title+keywords+description (72%) |
| concepts[field].local:field:earth-environmental | mapping · PANGAEA | connector:pangaea@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · PANGAEA | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · PANGAEA | connector:pangaea@1.0.0 | /metadata/dc/description |
| license | source · PANGAEA | connector:pangaea@1.0.0 | /metadata/dc/rights |
| publication_date | source · PANGAEA | connector:pangaea@1.0.0 | |
| spatial | source · PANGAEA | connector:pangaea@1.0.0 | |
| title | source · PANGAEA | connector:pangaea@1.0.0 | /metadata/dc/title |