Gene expression profiling of Escherichia coli isolates from aging colonies (Series 2)

Strains Y5 and Y6 were originally isolated from 3-week-old aging colonies, but RNA for transcriptomic profiling was extracted from 7-day-old colonies regrown under identical conditions. All samples were hybridized to the NimbleGen GPL9088 one-color microarray platform.

Processed values correspond to RMA-normalized, non-log2 intensities. Raw .pair files and the processed matrix for these samples are provided. These data complement Series 1 and constitute a second biological replicate (B2) for transcriptomic analysis of the parental strain and two evolved isolates carrying distinct mutations affecting regulatory and stress-response pathways in aging bacterial colonies.