Data Sheet 6_Peripheral exosomal miRNAs as candidate molecular indicators associated with acupuncture at ST36 in an LPS-induced neuroinflammation model.csv

Eight miRNA candidates were identified: five upregulated by LPS and showing a control-like pattern following ST36 (let-7d-5p, miR-103-3p, miR-107-3p, miR-451a, miR-7047-5p) and three downregulated by LPS and showing a control-like pattern following ST36 (miR-5128, miR-16-2-3p, miR-101a-5p). None overlapped with candidates from the original fold-change-based analysis. Functional enrichment of 1,596 predicted target genes revealed significant enrichment in MAPK, PI3K-Akt, and Wnt signaling pathways, with Gene Ontology analysis highlighting convergence on neuronal and synaptic processes.

Limma-based reanalysis reveals a distinct set of ST36-associated exosomal miRNAs overlooked by threshold-based approaches, providing exploratory candidate molecular indicators for further investigation of acupuncture's peripheral effects in neuroinflammation.</p>