Omics · study · 2026
Heterologous expression of interferon-stimulated genes reveals conserved anti-Toxoplasma properties between human and porcine cells
Listed in NCBI GEO
Toxoplasma is a zoonotic parasite that can cause life-threatening illness in humans and animals.
Description
The similarities in Toxoplasma pathogenesis between pigs and humans make pigs an attractive model for studying human toxoplasmosis. However, the mechanisms underpinning these similarities remain poorly understood.
Since interferon-gamma (IFNg) is crucial for immunity to Toxoplasma, we hypothesized that IFNg signaling pathways conserved between humans and pigs contribute to comparable Toxoplasma pathogenesis. Using a genetic gain-of-function strategy, we observed that human or porcine interferon regulatory factor 1 (IRF1) and indoleamine 2,3-dioxygenase 1 (IDO1) inhibited Toxoplasma replication in both homologous and heterologous human and porcine cells.
Read the rest (1 more)
IRF1-depleted porcine cells that failed to control Toxoplasma growth could be rescued by ectopic expression of human IRF1, which induces genes similar to those regulated by porcine IRF1. Additionally, the expression of either human or pig IRF1 dampened Toxoplasma-induced transcriptional remodeling of porcine genes. These findings highlight conserved IFN-induced anti-Toxoplasma mechanisms between pigs and humans and enhance the potential of pigs as large animal models for human toxoplasmosis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326196 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326196 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1444123 ncbi.nlm.nih.gov/bioproject/PRJNA1444123 ↗
project · from NCBI GEO
- PubMed 42004958 pubmed.ncbi.nlm.nih.gov/42004958 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Sus scrofa
- From keywords
- Life Sciences
- Inferred from text
- Animal production 70%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE326196 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3003 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9823 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |