Data for: Boosting Sensitivity of High-Dimensional Solid-State NMR of Proteins by Optimizing Coherence Transfers

While higher-dimensional approaches such as 4D and 5D correlation spectra are generally capable of exceeding the established molecular-weight limitations by ameliorating signal overlap, their intrinsic implications for sensitivity and measurement times required have limited these important prospects till-date.

Here we show that the extensive use of dedicated transverse-mixing optimal control pulses (TROPs) in high-dimensional experiments to enable concomitant transfer of complex signals along consecutive magnetization transfers can reduce the necessary measurement time by an order of magnitude. Owing to the multiplicative benefits of the enhancement for successive indirect chemical-shift dimensions, combined with non-linear benefits upon spectral reconstruction, the combination of non-uniformly sampled, higher-dimensional approaches with pulse sequences derived from concatenated TROPs presents itself as transformative with regard to feasibility of in-depth NMR characterization and hardware requirements.