Full-length Transcriptome Analysis of Artemisia tangutica Endemic to Qinghai-Tibetan Plateau

After the leaves were collected, they were quickly stored in liquid nitrogen. The voucher specimen (chen2019104) was deposited in the Qinghai Tibet Plateau Biological Herbarium (HNWP) of the Northwest Plateau Institute of Biology, Chinese Academy of Sciences. The total RNA of Artemisia tangutica leaves was extracted by Trizol method.

Agarose gel electrophoresis was used to check whether the extracted RNA was contaminated, and the RNA concentration was detected by nanodrop. The quality and integrity of RNA were evaluated by nanophotometer spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). For qualified RNA samples, Oligo DT was used as primer to reverse the target mRNA, and full-length cDNA was amplified by low cycle PCR.