Near-isotropic, aligned volume electron microscopy (eFIB-SEM) of mosquito stylet (jrc_mosquito-stylet-6)

It was processed using a reduced osmium-thiocarbohydrazide-osmium protocol with en bloc uranyl acetate staining, dehydrated through an acetone series, embedded in Eponate 12 resin, and polymerized at 60°C for two days. The selected sample was imaged by FIB-SEM at 8 nm isotropic resolution.<br><br><b>Contributions</b>: Sample provided by Hilda Amalia Pasolli (The Rockefeller University), prepared for imaging by Zhiyuan Lu (HHMI/Janelia) and imaged by Wei Qiu (HHMI/Janelia) and post-processing by Eric Trautman and Stephan Preibisch (HHMI/Janelia).<br><br><b>Acquisition ID</b>: jrc_mosquito-stylet-6<br><br><b>Voxel size (nm)</b>: 8 x 8 x 8 (x, y, z)<br><br><b>Data dimensions (µm)</b>:<b> </b>50.88 x 44.23 x 295.02 (x, y, z)<br><br><b>Imaging start date</b>: 2025-08-12<br><br><b>Imaging duration (days)</b>: 55<br><br><b>Landing energy (eV)</b>: 1200<br><br><b>Imaging current (nA)</b>: 2.00<br><br><b>Scanning speed (MHz)</b>: 0.500<br><br><b>Dataset URL</b>: <a href="data.janelia.org/IVV6Rg" target="_blank" rel="noreferrer">s3://janelia-cosem-datasets/jrc_mosquito-stylet-6/jrc_mosquito-stylet-6.zarr/recon-1/em/</a><br><br><b>Visualization Website</b>: <a href="openorganelle.janelia.org/datasets/jrc_mosquito-stylet-6" target="_blank" rel="noreferrer noopener">openorganelle.janelia.org/datasets/jrc_mosquito-stylet-6</a><br><br><b>Publication</b>:<b> </b>Xu et al., 2017</p>