Data · dataset · 2026
Identification and evaluation of a novel bioactive peptide QUB-2665 from the frog skin secretion of Phyllomedusa bicolor
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32638218.v1
In recent years, due to the increasing antibiotic resistance among microorganisms, the development of alternative drugs to antibiotics has become urgent.
Description
Peptide-based therapeutics have gained popularity as a promising direction in drug development, owing to their low resistance profile and other advantages. The skin secretions of frogs are well-known for their medicinal value, and peptides derived from these secretions have been found to possess various functions, including antibacterial activity.
In this thesis, a novel antibacterial peptide from the skin secretions of Phyllomedusa bicolor, named QUB-2665, was discovered and studied. Through molecular cloning, the QUB-2665 biosynthetic precursor sequence was obtained. Solid-phase peptide synthesis was used to synthesise the peptide.
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After that, the main structure of this new peptide was identified and purified using RP-HPLC and MALDI-TOF-MS. Utilising several microbes, minimum inhibitory concentration (MIC) assays were performed and the haemolytic activity assay was conducted using horse red blood cells to test QUB-2665. The results showed that QUB-2665 exhibited antimicrobial ability against E. coli with an MIC value of 16 mg/L and an MBC value of 16 mg/L, but showed no antibacterial activity towards S. aureus or yeast C. albicans.
QUB-2665 demonstrated strong haemolytic activity when the concentration was no less than 64 μM. Moreover, QUB had little effect on cancer cells. Based on these findings, it is evident that QUB-2665 has the potential to become an antibacterial drug.<br><br><i>Thesis is embargoed until 31 December 2028.</i>
Links
Where it is published
- DOI doi.org/10.17034/32638218.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 3 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32638218 | 9 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32638218 | 9 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32638218 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |