Supplementary material - Impact of Na+ permeation on collective migration of pulmonary arterial endothelial cells (Xu et al)

We found that permeation of extracellular Na + is necessary for efficient and cohesive migration of PAEC monolayers. The processes of wound healing displayed two phases: a fast healing phase within 5 hours followed by a slow-healing phase between 5 and 24 hours. Among the examined Na + channels, Na + permeation through ENaC produced prompt and largest effect on wound healing, Na + permeation through NCX produced small and delayed effect, Na + permeation through Orai1 produced small and short-term effect, and Na + permeation through TRPC1/4 produced insignificant effect.

Both extracellular Na + and Orai1 contribute to scratch wound healing, whereas only extracellular Na + but not Orai1 contributes to cell migration in the absence of injury.