Cystatin-B Degradome Foundation Atlas

Each fragment is characterized using physicochemical features relevant to mass spectrometry, proteomics, and computational peptide analysis.</p><p dir="ltr"><b>Mass Spectrometry and Peptide Metrics:</b></p><ul><li>Molecular weight</li><li>Mass-to-charge ratio (m/z)</li><li>Net charge</li><li>Isoelectric point (pI)</li></ul><p dir="ltr"><b>Biochemical and Biophysical Features:</b></p><ul><li>Hydrophobicity</li><li>Boman index</li><li>Instability index</li><li>Aliphatic index</li></ul><p dir="ltr">Together, these annotations provide a feature-rich computational reference for exploring the detectability, physicochemical behaviour, and potential analytical relevance of cystatin B-derived peptides.</p><h3 dir="ltr">Applications in Neurobiology, Biomarker Discovery, and Biomedical Research</h3><p dir="ltr">The Cystatin B Degradome Foundation Atlas can support a broad range of experimental and computational applications, including:</p><ul><li><b>Targeted Proteomics:</b> Identification and prioritization of candidate peptides for SRM/MRM and PRM mass-spectrometry assays.</li><li><b>Biomarker Discovery:</b> Exploration of cystatin B-derived peptide candidates associated with neurological disease, neurodegeneration, progressive myoclonus epilepsy, and altered proteostasis.</li><li><b>Discovery Proteomics:</b> Interpretation and annotation of cystatin B-derived signals detected in MS/MS datasets.</li><li><b>Degradomics:</b> Investigation of potential proteolytic processing patterns and fragment populations.</li><li><b>Neurobiology:</b> Computational exploration of cystatin B processing in neuronal function, cellular homeostasis, and disease-associated pathways.</li><li><b>Computational Biology:</b> Generation of reference features for peptide-level modelling, degradome analysis, and machine-learning applications.</li><li><b>Assay Development:</b> Prioritization of candidate cystatin B peptides for future experimental validation.</li></ul><h3 dir="ltr">Data Inclusion and Reproducibility</h3><p dir="ltr">The atlas provides a systematic computational representation of the <b>wild-type cystatin B degradome</b>, including the complete set of theoretically generated contiguous peptide fragments and their calculated physicochemical properties.</p><p dir="ltr">Importantly, this Foundation Atlas represents a <b>theoretical fragment space rather than a catalogue of experimentally confirmed cystatin B peptides</b>.

Experimental proteomic and mass-spectrometry studies will be required to establish which predicted fragments are generated and detectable under physiological or disease-associated conditions.</p><p dir="ltr">Future releases will expand the resource through the incorporation of additional cystatin B variants, disease-associated mutations, experimentally validated cleavage products, and complementary experimental proteomics data.</p>