Table · dataset · 2025
Spaceflight influences gene expression, photoreceptor integrity, and oxidative stress related damage in the murine retina (RR-9)
Listed in NASA Data Portal
Extended spaceflight has been shown to adversely affect astronaut visual acuity.
Description
The purpose of this study was to determine whether spaceflight alters gene expression profiles and induces oxidative damage in the retina. Ten week old adult C57BL/6 male mice were flown aboard the ISS for 35 days and returned to Earth alive.
Ground control mice were maintained on Earth under identical environmental conditions. Within 38 (+/−4) hours after splashdown, mice ocular tissues were collected for analysis. RNA sequencing detected 600 differentially expressed genes (DEGs) in murine spaceflight retinas, which were enriched for genes related to visual perception, the phototransduction pathway, and numerous retina and photoreceptor phenotype categories.
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Twelve DEGs were associated with retinitis pigmentosa, characterized by dystrophy of the photoreceptor layer rods and cones. Differentially expressed transcription factors indicated changes in chromatin structure, offering clues to the observed phenotypic changes. Immunofluorescence assays showed degradation of cone photoreceptors and increased retinal oxidative stress.
Total retinal, retinal pigment epithelium, and choroid layer thickness were significantly lower after spaceflight. These results indicate that retinal performance may decrease over extended periods of spaceflight and cause visual impairment. This dataset is from the micro-computed tomography, immunostaining, and histology assays.
Links
Get the data
- Resource osdr.nasa.gov/bio/repo/data/experiments/OS-140 ↗
download · from data nasa gov
- Resource osdr.nasa.gov/bio/repo/data/missions/SpaceX-12 ↗
download · from data nasa gov
- Resource purl.bioontology.org/ontology/NCBITAXON/10090 ↗
download · from data nasa gov
- Resource osdr.nasa.gov/bio/repo/data/studies/OSD-557 ↗
download · from data nasa gov
- Resource nlsp.nasa.gov/view/lsdapub/lsda_experiment/f30954b4-0ed9-5d25-896d-5eed50068… ↗
download · from data nasa gov
Where it is published
- NASA Data Portal dataset page data.nasa.gov/dataset/spaceflight-influences-gene-expression-photoreceptor-i… ↗
landing page · from data nasa gov
- DOI doi.org/10.26030/yv31-1a54 ↗
DOI / persistent id · from data nasa gov
Catalogue records · 1
- CKAN API data.nasa.gov/api/3/action/package_show?id=f7f6a3d6-6481-46b8-a639-01ef7e1b2… ↗
metadata API · from data nasa gov
Topics
- From keywords
- Astronomy & Astrophysics · Earth & Environmental Science · Life Sciences · Ocean & Atmospheric Science · Physics
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Data Portal | f7f6a3d6-6481-46b8-a639-01ef7e1b2c5c | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:astronomy | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:physics | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| description | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /notes |
| license_text | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| publication_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| title | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /title |
| updated_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 |