Data · dataset · 2026
Study on the Mechanism of Polygonatum cyrtonema Hua Polysaccharide 1 in Improving Obesity by Modulating Calcium Signaling Pathways
Listed in ScienceDB
This study integrated in vitro and in vivo models with transcriptomic analysis to investigate the effects and potential mechanisms of Polygonatum cyrtonema Hua polysaccharide 1 (PCP1) against obesity.
Description
In vitro, cocktail-induced differentiation of 3T3-L1 preadipocytes was treated with PCP1 at 25, 50, or 100 μg/mL and evaluated using Oil Red O staining, RT-qPCR, and pharmacological inhibition or activation of calcium signaling.
PCP1 concentration-dependently reduced lipid droplet accumulation and downregulated Pparg and Cebpa expression. Experiments using 2-APB and ionomycin indicated that its inhibitory effect on adipocyte differentiation was at least partly dependent on calcium signaling. In vivo, ob/ob mice were assigned to model, semaglutide, and PCP1 groups, with wild-type mice serving as normal controls, and treated for 8 weeks.
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PCP1 reduced body weight and the weights of subcutaneous adipose tissue, epididymal adipose tissue, and liver; improved fasting hyperglycemia., blood lipids disorders, glucose intolerance, and insulin resistance; alleviated hepatic steatosis, lipid deposition, and subcutaneous adipocyte hypertrophy; and decreased ALT and AST levels. PCP1 also increased UCP1 protein expression in brown adipose tissue, whereas no evident abnormalities were observed in BUN, Cr, or the histopathology of the heart, spleen, lung, and kidney.
Transcriptomic analysis identified 649 differentially expressed genes, mainly enriched in calcium signaling, fatty acid metabolism, fatty acid biosynthesis, and thermogenesis. RT-qPCR confirmed that PCP1 downregulated the calcium signaling-related genes Itpr3, Vdac3, Flt1, and Slc8a1; upregulated the fatty acid β-oxidation- and thermogenesis-related genes Sirt1, Ucp1, Ppara, and Pgc-1α; and suppressed the lipogenesis-related genes Fasn, Dgat2, Scd1, and Cd36.
Collectively, PCP1 may ameliorate obesity and associated metabolic abnormalities by modulating calcium signaling, promoting fatty acid β-oxidation and thermogenesis, and suppressing lipid synthesis, supporting its development as an anti-obesity functional food ingredient.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.nprd.00388 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Heart 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.nprd.00388 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[anatomy].local:anatomy:heart | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |