Spatial characterization of microbial communities on multi-species leafy greens grown simultaneously in the Veggie vegetable production systems on the International Space Station VEG-03D

This studies also stresses the food safety component of fresh crops grown on the ISS. The VEG-03D technology demonstration was completed achieving simultaneous multi-species plant growth within a Veggie unit on the International Space Station (ISS).

Microbiological characterization using molecular and culture-based methods was performed on leaves and roots from two harvests of three leafy greens, red romaine lettuce (Lactuca sativa cv. ‘Outredgeous’); mizuna mustard, (Brassica rapa var japonica); and green leaf lettuce, (Lactuca sativa cv. Waldmann’s) and associated rooting pillow components and Veggie chamber surfaces. Culture based enumeration and pathogen screening indicated the leafy greens were safe for consumption.