Data · dataset · 2026
A gut commensal bacterium enhances orthoflavivirus E protein thermostability to enable fecal-oral transmission
Listed in ScienceDB
Orthoflaviviruses are typically maintained in nature through mosquito borne cycles.
Description
How some strains acquire the ability to transmit via direct, non vector routes in vertebrate hosts remains a key evolutionary question. Here, using Tembusu virus (TMUV) in an avian model, we identify a molecular mechanism that explains its cross species transmission from mosquitoes to avian populations.
We demonstrate that avian hosts are highly susceptible to TMUV via the oral (intragastric) route, establishing an efficient fecal oral transmission pathway that bypasses the mosquito vector. Infection induces typical clinical signs with 40% mortality. Viral replication occurs in the brain and spleen, with the cloaca serving as the primary shedding site for up to 28 days—yielding significantly higher titers than pharyngeal swabs and indicating prolonged environmental contamination.
Read the rest (3 more)
Even a low dose (10³ TCID50) establishes infection, primarily restricted to the duodenum. Maternal antibodies prevent mortality but do not block viral replication. Critically, we uncover the mechanism that enables this cross species transition.
Using single round infection particles and E swapped chimeric viruses (CQ/MM E and MM/CQ E), we show that specific interactions between a naturally isolated gut commensal bacterium (Enterococcus hirae 293) and the viral envelope (E) protein enhance viral thermostability. This bacterium mediated stabilization allows the virus to retain infectivity within the gastrointestinal tract and the external environment—conditions fundamentally different from the mosquito vector environment.
Thus, our results directly explain how an ancestral mosquito adapted orthoflavivirus can overcome the thermal and enzymatic barriers of the avian gut, enabling a switch from vector borne to fecal oral transmission in an avian host.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.41644 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.41644 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |