Genetic data from 4 leafless vanilla populations

We removed 15 cm of stems or young buds and preserved them in silica gel, obtaining a total of 100 individuals for the four populations. DNA extraction was performed using Qiagen's DNEASY® plant kit for all 100 specimens.

Seven microsatellite markers developed by Gigant et al (2011) were used to amplify and genotype each individual. Polymerase chain reaction (PCR) with fluorescently-labeled primers was performed in a total volume of 25 μl using a Promega kit (Promega, 2007). The mixture contained 12.5 μl of GoTaq Colourless Master Mix 2X, 2 μl of DNA solution (5 < DNA < 250 ng), 0.4 μM of each primer and 8.5 μl of nuclease-free water.