Transcriptome analysis of Vigna radiata leaves in response to direct and pre-conditioned salt stress [RNA-Seq]

Two treatments were applied: T1 (pre-conditioned with 100 mM NaCl for 24 h, recovered for 48 h, then re-exposed to 100 mM NaCl) and T2 (direct exposure to 100 mM NaCl without preconditioning). Samples were collected at 0, 1, 3, 6, 12, 24, and 48 h after the onset of the final salt treatment from the first fully expanded true leaves (n=3 per time point per treatment).

Plants were grown under controlled conditions (25°C, 12,000 lux, 16h/8h light/dark, 70% RH) in 1/2 MS nutrient solution. The 0 h time point in T2 represents naive control, while in T1 it reflects residual effects after recovery. Data aim to reveal transcriptional reprogramming under salt stress and preconditioning.