Potential Association of certain microRNA gene polymorphisms with recurrent pregnancy loss susceptibility in Saudi women

This case control study investigated six miRNA polymorphisms (miR-10-A>T, miR-125-G>A, miR-146a-C>G, miR-149-T>C, miR-323b-C>T, miR-499-A>G) in 50 Saudi women with idiopathic (≥ 2 losses) and 50 matched controls (≥ 1 live birth, no loss history) using PCR Sanger sequencing.

Significant associations were found for heterozygous genotypes of miR-146a-C>G (OR=2.29, 95% Cl:1.02-5.18, *p*=0.046) and miR-149-T>C (OR=2.67, 95% Cl:1.08-6.61, *p*=0.034) with higher prevalence in RPL patients versus controls, while other polymorphisms showed no significant association (*p*>0.05). These results suggest miR-146a and miR-149 can contribute to RPL susceptibility in Saudi women, highlighting their potential as population-specific genetic biomarkers and underscoring the need for further research into miRNA-mediated pregnancy maintenance mechanisms.