Data for: “Single mutation in iolT1 in ptsG deficient C. glutamicum enables growth boost in xylose containing media”

The data uploaded here supplement the publication titled “Single mutation in iolT1 in ptsG deficient C. glutamicum enables growth boost in xylose containing media” Through rational strain design, continuous adaptive laboratory evolution (ALE) and whole-genome sequencing (WGS), we have identified a single point mutation in the iolT1 gene, leading to an amino acid exchange from alanine to proline in IolT1 that alters cellular uptake capacity and growth rate.

Biomass specific glucose uptake rate was increased by 83 % and xylose uptake by 20 %. The overall growth rate of the strain was improved by 35 % in mixed glucose-xylose media. To our knowledge, this study is the first report of an engineered engineered IolT1 variant that significantly enhances substrate uptake in PTS-independent C. glutamicum.