<p>Genetic disruption of the <i>mgsA</i> gene (<i>bb0364</i>).</p>

(B) Immunoblot of total cell extracts from wild-type (WT), ∆<i>mgsA</i> and <i>mgsA</i>⁺ strains using anti-MgsA and anti-FlaB antibodies (loading control).

(C) Anti-MG immunoblot of glycated proteins (upper panel) from total cell extracts of wild-type (WT), ∆<i>mgsA</i> and <i>mgsA</i>⁺ strains in RPMI (nutrient stress), RPMI with 100 µM glycerol or 100 µM methylglyoxal (MG) for 24 h at 35°C. (D) Anti-MG immunoblot of glycated proteins (upper panel) from total cell extracts of wild-type (WT), ∆<i>mgsA</i> and <i>mgsA</i>⁺ strains in RPMI with 0.4% neopeptone or BSK without BSA and without RS (BSK -BSA -RS) for 24 h at 35°C.</p>