Supplementary figures and tables for “Developmental competence and molecular signatures of cloned and recloned embryos derived from (non-) transgenic cloned buffalos” by Yang et al., Animal Genetics

The data include:Fig. 1 – Generation of transgenic cloned buffalo: (a) stably transfected ear fibroblasts, (b) SCNT embryo derived from transfected fibroblasts, (c) transgenic buffalo calf, (d) ear tissue of transgenic vs. normal buffalo under UV light, (e) ear tissue of offspring.Fig. 2 – Transgenic buffalo and its offspring: (a) adult transgenic buffalo (3.5 yr), (b) first-born offspring (non-EGFP), (c) second-born offspring (EGFP-positive).Fig. 3 – Western blot analysis of EGFP expression in newborn, adult, and offspring transgenic buffalo.Fig. 4 – Fluorescence microscopy of EGFP expression in ear fibroblasts (newborn, adult, offspring).Fig. 5 – Relative mRNA expression levels of Oct-4, Cdx2, Gata3, E-cadherin, β-catenin, and Nanog in IVF, normal SCNT, and transgenic SCNT blastocysts.Fig. 6 – Relative mRNA expression levels of telomere-related genes (TERT, TERC, Terf1, Terf2) in original fibroblasts (OF), newborn transgenic fibroblasts (NTF), and adult transgenic fibroblasts (ATF).Table 1 – Real-time PCR primer sequences (including product size and accession numbers).Table 2 – SCNT efficiency (fusion, cleavage, blastocyst rates) of reconstructed embryos derived from different cell types (OF, CF, TF, NTF, ATF, IVF).Table 3 – Pregnancy and abortion rates of non-transgenic and transgenic embryos.All files are provided as high-resolution source images (PNG/TIFF) and tables (CSV/PDF) to ensure full transparency and reproducibility of the published results.