TROPIC: An Integrative Approach Aimed at Identifying Drug Targetable Human- Parasite Interactions

Cuesta-Astroz through the pipeline they developed for orthology-based host-pathogen protein-protein interactome identification (Cuesta-Astroz, Santos, Oliviera, & Jensen, 2019) . The Cuesta-Astroz Team reviewed these interaction data through proteome filtering to prioritize potentially interacting proteins.

In summary, the pipeline consists of the following steps: filtration of parasite proteins by secretome and membrane localization (based on whether the parasite is unicellular or multicellular), filtration of host proteins by tissue expression, and using STRING to perform an orthology-based mapping of intraspecies interactions to host-parasite pairs. The pipeline concentrates on domain-domain and domain-motif interactions of the protein-protein complexes yielded by the previous filters to prioritize the protein-protein interactions that are more possibly physically obtainable.