Identification and Characterization of MicroRNAs in female 0edaleus asiaticus(Orthoptera:Oedipodidae)

Here, we performed small RNA sequencing on female O. asiaticus at three developmental stages: 3-day-old third-instar nymphs, 3-day-old fifth-instar nymphs, and newly emerged adults. We identified 365 miRNAs (291 known, 74 novel), with 75 differentially expressed across stages.

Target prediction for 43 differentially expressed miRNAs yielded 42,773 putative targets.GO and KEGG enrichment analysis were performed on the target gene set to reveal the biological processes, cell components, molecular functions and metabolic signaling pathways involved in these target genes. This study presents the first miRNA expression profiling of O. asiaticus, providing a valuable resource for future growth and development regulatory mechanism research.