Time-course bulk RNA-seq dataset of mouse brain and liver following PLX3397 treatment

Adult male C57BL/6J mice were treated with a 600 ppm PLX3397 diet for 0, 3, 7, or 21 days (n = 3 per group). Brain and liver tissues were collected at each time point, and RNA libraries were sequenced on the Illumina NovaSeq 6000 platform.Raw sequencing reads were quality-filtered using fastp, aligned to the mouse reference genome (GRCm38) using HISAT2, and quantified with featureCounts to generate raw count and FPKM expression matrices.

Raw count data were used for differential expression analysis with DESeq2, whereas FPKM values were used for gene expression visualization.The dataset comprises 24 RNA-seq libraries together with raw FASTQ files, raw count matrices, FPKM expression matrices, differential expression results, and sample metadata. All data are provided in standard FASTQ and CSV formats and can be readily analyzed using commonly available bioinformatics tools.