Differential gene expression in the rat hippocampus and dorsal raphe nucleus following chronic low level exposure to the pesticide diazinon

Probe sets (78,963) were detected in all samples (20% and 90% bound of the fluorescence intensity of the chip). For the detection of gene expression changes, an ANOVA was applied with a cut off at a false discovery rate p < 0.05. All up-regulated and All down-regulated worksheets = probe sets significantly detected in samples.

Significant worksheet = genes significantly different between vehicle and diazinon treated animals function, pathway and network worksheets = Ingenuity pathway analysis was conducted on genes that were significantly altered to generate functions and canonical pathways using the Ingenuity Systems reference set that were most significant to the data set. The p value was determined by the probability that the association between the genes in the dataset and the function or canonical pathway is explained by chance alone.

The significance of the association between the data set and the function or canonical pathway was calculated using Fischer’s exact test. Networks were generated using the IPA Network Generation Algorithm and scored based on the number of network eligible molecules they contained from the dataset. Funded by Health Protection Agency and a Department of Health Policy Research Programme grant (UK).