Data sets on soil bacteria, enzyme activities, physical and chemical properties for different land use types and soil depths

The data contain three types of core indicators: (1) Soil physicochemical properties: including pH, bulk density (BD), soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), quick-acting phosphorus (AP), and quick-acting potassium (AK) were measured by potentiometric, ferrocyclic, and oxidative potassium dichromate methods, respectively; (2) Enzyme activity data: six enzymes related to C/N/P cycling (α-glucosidase AG, β-glucosidase BG, etc.) were measured using microplate fluorescence; (3) Microbial community data: 16S rRNA genes VG, β-glucosidase BG, etc., were measured by Illumina PE300 platform. glucosidase AG, β-glucosidase BG, etc.), which were detected using the microtiter plate fluorescence method; (3) Bacterial community data: bacterial community composition and diversity data (e.g., relative abundance of phyla such as Chloroflexi, Actinobacteriota, etc.) were obtained by sequencing the V4-V5 region of the 16S rRNA gene via the Illumina PE300 platform.

The data collected were systematically analyzed through the above methodology to achieve the following objectives: (i) to study the effects of land use and soil depth on bacterial community composition and enzyme activities; (ii) to elucidate the relationship between soil physicochemical properties and bacterial communities as well as enzyme activities; and (iii) to explore the functional roles of enzymes in nutrient cycling and to predict bacterial functional profiles.