Supplemental Table S1 - S4

Comparison is shown for extracellular Ca 2+ concentration of 2 mM versus 0.1 μM. These data are the mean ± standard deviation from five different independent experiments. The data is shown in Fig. 4. Supplemental Table S3: Comparison of baseline PMF intensity of (A) PAECs cultured on 30 kPa versus on 1.25 kPa hydrogels, and (B) PAECs cultured in 0.1 μM extracellular Ca 2+ versus 2 mM extracellular Ca 2+ .

On the left is the comparison of PAECs on 1.25 kPa hydrogel versus those on 30 kPa hydrogel. On the right is the comparison of PAECs in normocalcemic (2 mM Ca 2+ ) versus hypocalcemic (0.1 μM Ca 2+ ) culture medium. These data are the mean ± standard deviation from five different independent experiments.

These two tables present two different comparisons of the same four sets of experiments shown in Fig. 5 and 6. Supplemental Table S4: Comparison of the range and duration of ΔPMF in PAECs cultured on 30 kPa versus 1.25 kPa hydrogels. The first column shows a comparison of the range of ΔPMF between PAECs cultured on 1.25 kPa hydrogels versus those cultured on 30 kPa hydrogels.

The second column shows a comparison of the duration of ΔPMF between PAECs cultured on 1.25 kPa hydrogels versus those cultured on 30 kPa hydrogels. These data are the mean ± standard deviation from five different independent experiments. These data are shown in Figs. 5 and 6.