Data · dataset · 2026
<p>Raw images of gels and blots.</p>
Listed in ZivaHub and Deakin Research Online and DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.1371/journal.pone.0359197.s011
<div><p>Ribosomal proteins, because of their RNA-binding capacity, may engage various cellular RNAs and fulfill non-ribosomal roles.
Description
Previously, we and others described the intergenic regulation mediated by splicing of <i>RPL22</i> paralogs in <i>Saccharomyces cerevisiae</i>. Here, we prepared a panel of <i>RPL22A/B</i> intronic mutants with respect to their RNAfold-predicted features and analyzed their properties.
We tested splicing efficiency and Rpl22-intron binding using an intron-containing reporter and a yeast three-hybrid system, respectively. We found that the splicing of <i>RPL22</i> introns can be inhibited by stabilizing a predicted stem as part of a particular type of conformation (I structure). Stabilizing the formation of an alternate stem (P structure) permitted splicing.
Read the rest (2 more)
Binding of the Rpl22 protein to the intron led to enhanced splicing inhibition in WT and several of the mutants, which we interpret as stabilization of the I structure. Mutagenesis identified both the main and alternative 5’ss and additional stem loops as part of the regulatory mechanism. The inhibitory conformation of the intron did not prevent recognition of the 5’ss and branch point, but rather stalled splicing at a later stage, before the first catalytic step.
We conclude that the structural ensemble of the <i>RPL22</i> pre-mRNA behaves as an allosteric switch that responds to Rpl22 concentration.</p></div>
Links
Where it is published
- DOI doi.org/10.1371/journal.pone.0359197.s011 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Cancer · Cancer · Cancer · Cancer · Chemistry · Chemistry · Chemistry · Chemistry · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Genetics · Genetics · Genetics · Genetics · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Medicine & Health · Medicine & Health · Medicine & Health · Medicine & Health · Microbiology · Microbiology · Microbiology · Microbiology · Saccharomyces cerevisiae · Saccharomyces cerevisiae · Saccharomyces cerevisiae · Saccharomyces cerevisiae · Virology · Virology · Virology · Virology
- Inferred from text
- Image 65%
Provenance · 4 source records, 42 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34047602 | 8 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/34047602 | 8 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/34047602 | 8 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/34047602 | 8 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:cancer | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:cancer | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:cancer | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[field].anzsrc:field:310706 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:310706 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:310706 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:310706 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:group:3105 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3107 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].local:field:chemistry | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:4932 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| concepts[organism].NCBITaxon:4932 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| concepts[organism].NCBITaxon:4932 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| concepts[organism].NCBITaxon:4932 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |