Global peatlands - microbiological data

Nitrous oxide fluxes, microbiological (sequencing, qPCR) and soil physicochemical parameters data of peatlands globally We sampled gas and soil in 29 regions throughout the A (rainy tropical), C (temperate), and D (boreal) climate types of the Köppen classification from six continents during the vegetation period between August 2011 and June 2018, following a standard protocol. Within the sites, we established 1–4 stations 15–500 m apart to maximize the captured environmental variation.

Each of the 196 stations were equipped with 3–5 opaque PVC 65 L truncated conical chambers 1.5–5 m apart and an observation well (perforated, 50 mm diameter PP-HT pipe wrapped in geotextile; 1 m in length). From each of the 645 chambers, N2O fluxes were measured following the static chamber method using PVC collars (0.5 m diameter, installed to 0.1 m depth in soil). Soil samples of 150–200 g were collected from the chambers at 0–10 cm depth after the final gas sampling, and transported to laboratories in Tartu, Estonia.