<p>Comparison of IFNγ induced gene expression in donor-derived keratinocytes and matched donor-derived fibroblasts.</p>

Data is represented as dCt, which is calculated by subtracting the Ct value of β-actin from the Ct of induced genes. All Ct values were acquired directly from qPCR with a maximum Ct threshold of 35. <b>(B)</b> Fold change in <i>CXCL10, CCL5, IL15, IL12A</i>, and <i>IFI16</i> expression in primary donor keratinocytes and matched-donor fibroblasts 4 hours post treatment with IFN-γ.

Fold change calculated as relative to mock expression, normalized to β-actin, calculated using the ΔΔCt method. <b>(C)</b> IFNγ-induced gene expression at 4- and 24-hours post-treatment in keratinocytes and donor-matched fibroblasts (n = ≥3 donors with 3 replicates per donor). Symbols represent the geometric mean of each individual donor. ^*<i>P</i> < 0.05, ^** <i>P</i> < 0.01, ^*** <i>P</i> < 0.001, NS, not significant; linear mixed models were used to analyze fold change in gene expression.</p>