Single-cell Multi-modal Integrative Analyses Highlight Functional Dynamic Gene Regulatory Networks Directing Human Cardiac Development, Holman et al.

Fig. 1B contains 2D and 3D images for Ki67 (Channel 1) and TNNT2-mKate2 (Channel 2) Fig. 1C contains 2D and 3D images for N-Cadherin (Channel 1) and TNNT2-mKate2 (Channel 2) Fig. 1D contains 2D and 3D images for N-Cadherin (Channel 1), Connexin-43 (Channel 2), and TNNT2-mKate2 (Channel 3) Fig. 1F contains 2D and 3D images for TMRE (Channel 1) Fig. 5A contains original DNA gel to show PCR genotyping that confirmed three hPSC HSF1 KO lines (versus the control hPSC line).

Lanes 1 and 6 are ladder, Lane 2 is control, and Lanes 3-5 are HSF1 KO clines. Fig. 5D contains 3D Control and KO-2 images for ROS (Channel 1), TNNT2-GFP (Channel 2), TMRE (Channel 3), and DAPI (Channel 4) Fig. S1B contains 2D image for DAPI (Channel 1), TNNT2-mKate2 (Channel 2), and TNNT2 antibody (Channel 3) Fig. S5E contains original Western Blot for HSF1 and GAPDH. One blot in bright field to show ladder and one blot in chemiluminescence to show HSF1 and GAPDH.