A single-cell time-series atlas of endothelial cell embryonic development

Here, we generated a time-series endothelial resource covering the entirety of mouse embryonic development, including 26 time points and 8 organs. Time-series multiorgan comparison revealed emergence timing and lineage trajectory of organotypic ECs together with organ-specific genes and pathways.

Using these resources, we found that most ECs showed distinguishable organ specificity before late-gestation. The organotypic EC-enriched genes were associated with vascular function in the organs. Human and mouse pulmonary ECs underwent an evolutionarily conserved transcriptional transition.