Raw data from transcriptome sequencing of A549/DDP cells

Each group included three biological replicates.The experimental procedure was as follows: A549/DDP cells were expanded and seeded into 24-well plates. Transfection complexes were prepared using Opti-MEM (500 μL), Lipofectamine 2000 (20 μL), and either LINC00511 siRNA (50 μL) or an overexpression plasmid (15 μg).

Cells were transfected after overnight incubation at 37 ℃ in a 5% CO₂ atmosphere. Twenty-four hours post-transfection, cells received designated treatments according to their assigned groups: final concentrations of DDP at 24 μM and β-Elemene at 40 μg/mL. Following an additional 24-hour incubation under standard culture conditions (37 ℃, 5% CO₂), cells were harvested, washed with PBS, lysed thoroughly in TRIzol reagent, snap-frozen in liquid nitrogen, and submitted to Wuhan Lingsi Biotechnology Co., Ltd. for transcriptome sequencing.