Data · dataset · 2026
<p><i>Pf</i>ATG18 is required for DV maintenance and hemoglobin delivery.</p>
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<p>Synchronized parasites were treated with DMSO or RAP from the ring stage onward and analyzed at 36 hours post-invasion. <b>(A,B)</b> Loss of <i>Pf</i>ATG18 causes fission of the DVM without compromising its barrier function.
Description
Shown are fluorescently tagged CRT <b>(A)</b>, and PM2 <b>(B)</b>. <b>(C,D)</b> <i>Pf</i>ATG18-deficient parasites harbor two distinct populations of host cell cytosol-containing vesicles. <b>(C)</b> Localization of HRP2, a parasite protein exported into the host cell and subsequently reinternalized.
Cyan arrowheads: bright compartments; yellow arrowheads: dim compartments. (D) <i>atg18:loxP + crt-mNG</i>-infected erythrocytes were treated with 5-aminolevulinic acid (5-ALA) to induce synthesis of fluorescent protoporphyrin IX (PPIX) in the host cell. White arrowheads: PPIX-positive compartments lacking CRT. <b>(E-G)</b> SBF-SEM reveals DV fragmentation and accumulation of spherical compartments in <i>Pf</i>ATG18-deficient parasites.
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Representative sections (E) and 3D reconstructions (F) highlight relevant organelles. <b>(G)</b> Z-series of a spherical compartment in a <i>Pf</i>ATG18-deficient parasite. Green: hemozoin-containing compartments; orange arrowheads: spherical compartments; red arrowheads: membrane invagination overlain by a second limiting membrane. Scale bars: 1 µm. <b>(H)</b> <i>Pf</i>ATG18-deficient parasites accumulate compartments positive for the late endosomal marker Rab7. <b>(I,J)</b> Loss of <i>Pf</i>ATG18 results in accumulation of undigested hemoglobin.
Parasites released by saponin lysis were analyzed by SDS-PAGE, and hemoglobin (Hb) was visualized by Coomassie staining (I) or Western blotting <b>(J)</b>. Ery: erythrocyte extract. <b>(K)</b> Loss of <i>Pf</i>ATG18 reduces hemozoin formation, as quantified by polarization microscopy. Individual and mean values; paired <i>t</i>-test; n = 99 parasites, 3 experiments. *, <i>p</i> < 0.05.
Scale bars: 5 µm unless noted otherwise. Source data are provided as a <a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014608#ppat.1014608.s015" target="_blank">S3 File</a>.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.ppat.1014608.g003 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Biochemistry and cell biology · Earth & Environmental Science · Genetics · Infectious diseases · Life Sciences · Medicine & Health · Microbiology
- Inferred from text
- Microscopy 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34017854 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
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| concepts[field].anzsrc:field:320211 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:group:3101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3107 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:microscopy | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |