Integrative genomic, phylogenetic, and transcriptomic analyses reveal temperature-responsive transient receptor potential (TRP) genes in Pacific abalone Haliotis discus hannai

Spatial expression profiling revealed distinct tissue-specific patterns: TRPC-, TRPM-, and TRPP-like genes were enriched in sensory organs (i.e., the eyes and tentacles), whereas TRPM- and TRPV-like genes were expressed predominantly in respiratory and metabolic tissues (i.e., the gills and hepatopancreas). Under acute thermal stress, RNA sequencing and real-time quantitative PCR identified several thermoresponsive TRP paralogs, including TRPA1- and TRPV-like genes, exhibiting distinct transcriptional regulation.

These results elucidate the evolutionary complexity and functional diversification of TRP channels in marine gastropods, and highlight the potential role of these molecules in thermal sensing and adaptation. This study provides a molecular framework for understanding TRP-mediated environmental responses in mollusks, contributing to broader insights into marine invertebrate resilience under climate change.