Sequential enhancer sequestration dysregulates recombination center formation at the IgH locus

Figure 1 and S3 show that Eµ loops to an alternate site close to the 3’-most functional VH gene segment, VH81X, upon disruption of its interaction with IGCR1. Figure 2 and S4 support that Eu-VH81X loop requires both YY1 and CTCF.

Figure 3 and S5 demonstrate that DFL16.1 utilization is reduced on IGCR1-deficient alleles. Figure 4 and S6 shown that VH (VH713 and VHQ52) recombination is greatly induced and Eu-dependent on IGCR1-deficient alleles. Figure S7 show the verification of IGCR1 deleted cell line.