Nup133 is required for nuclear pore basket assembly and dynamics in embryonic stem cells. B. Souquet et al.

Each folder encompasses a general montage, in which the areas used for Figure 4C are indicated in red, as well as 5 sub-folders corresponding to the original 16-bit images (each include the ECL signal and the image of the membrane to visualize the molecular markers). For each pull-down experiment, the same samples were loaded on both nitrocellulose (that was stained with Ponceau) and PVDF membranes. - The top of the nitrocellulose membrane was first probed with Nup107, cut, and reprobed with anti-Nup133 His-Nup153 pull-down (nitro I or ii) Nup107 His-Nup153 pull-down (nitro I or ii) Nup133 (rehybr after Nup107) - The bottom of the nitrocellulose membrane was probed with anti-GAPDH antibody His-Nup153 pull-down (nitro i) GAPDH - The PVDF membrane was first cut and probed with Tpr, Nup96 and Nup85 His-Nup153 pull-down (PVDF i)Tpr-Nup96-Nup85 - The part of the PVDF membrane probed wiuth Nup98 was then reprobed with anti Nup98 His-Nup153 pull-down (PVDF i)_Nup98 (rehyb from Nup96)