X-ray diffraction images for the protein Mtb-AnPRT crystallised in the presence of the ligand

X-ray diffraction images were collected at the Australian Synchrotron on the MX1 beamline on March 25th, 2015. Regardless of the manner the ligand was introduced, both datasets were found to process with the same space group (C2) and could be subquently solved using molecular replacement.

The protein Mycobacterium tuberculosis anthranilate phosphoribosyltransferase (Mtb-AnPRT) used in this study had previously been found to process in four different space groups (e.g. P21, C2, P21212, and P212121). The ligand utilised in these experiments was a Mtb-AnPRT inhibitor, 2-(2-carboxyphenylamino)-5-(3-phosphonopropoxy)benzoic acid (annotated as 8i). This inhibitor is similar to one of the enzyme's substrates -- i.e. bianthranilate-like.