Identification and functional study of the bioactive peptide QUB-2830 from the skin secretion of the blue-side leaf frog, Agalychnis annae

In antimicrobial assays, QUB-2830 showed significant antimicrobial activity against a Gram-negative bacterium, Escherichia coli (E. coli ATCC CRM8739), a Gram-positive bacterium, Staphylococcus aureus (S. aureus ATCC CRM6538), and a yeast, Candida albicans (C. albicans ATCC CRM10231). For S. aureus, the minimum inhibitory concentration (MIC) was 1 μM, for E. coli, the concentration was 2 μM, for C. albicans, it was 4 μM. In terms of the MTT assay, QUB-2830 was shown to have antiproliferation activity against NCI-H838 cancer cells at a concentration of 10-4 M. In a haemolysis assay, QUB-2830 showed haemolytic activity at concentrations between 16 ~ 128 μM. In summary, QUB-2830 exhibited a remarkable antimicrobial ability and may be expected to become a safe antimicrobial drug in the future after its haemolysis activity is reduced through structural modification.<br><i><br>Thesis embargoed until 31 December 2026</i>.