Data · dataset · 2015
Generation and Application of a Novel InsP 3 R 1 Mono-Antibody from Mouse
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Inositol 1, 4, 5-Trisphosphate Receptor (InsP 3 R) is an intracellular Ca 2+ release channel, which widely participates in cellular processes.
Description
Three isoforms of InsP 3 R were identified as InsP 3 R 1 , InsP 3 R 2, and InsP 3 R 3 . They share 60–0% protein sequence homology and form a channel in a manner of homotetramer or heterotetramer.
Several InsP 3 R isoform-specific rabbit antibodies have been generated to distinguish their localization and functions. However, there is no report of such a valid antibody raised from other species. In his article, we prepare a mouse monoclonal antibody against a synthetic peptide with rat InsP 3 R 1 -specific carboxyl terminus sequence.
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This monoclonal antibody of InsP 3 R 1 (R1-mAb) was purified and characterized as IgG2b, which can recognize InsP 3 R 1 by Western-blot (WB) analysis and immunoprecipitate (IP) InsP 3 R 1 from moue brain lysate tested. Applied in immunofluorescent (IF) and immunohistochemical (IHC) assays, this antibody and rabbit polyclonal antibody could give the consistent results in SH-SY5Y cells, human sperm, and mouse brain paraffin section.
In summary, we generate a mouse InsP 3 R 1 -specific IgG 2b antibody identifying InsP 3 R 1 in WB, IF, IHC, and IP analysis, which provides a possible choice for detection of InsP 3 R 1 , especially in application of co-localization analysis with other InsP 3 R isoforms or other proteins.
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Where it is published
- Repository landing page tandf.figshare.com/articles/dataset/Generation_and_Application_of_a_Novel_InsP_su… ↗
landing page · from DataCite
- Repository landing page tandf.figshare.com/articles/dataset/Generation_and_Application_of_a_Novel_InsP_su… ↗
landing page · from DataCite
- DOI doi.org/10.6084/m9.figshare.1275093 ↗
DOI / persistent id · from DataCite
- DOI doi.org/10.6084/m9.figshare.1275093.v2 ↗
DOI / persistent id · from DataCite
Documentation and papers
- Creative Commons Attribution 4.0 International creativecommons.org/licenses/by/4.0/legalcode ↗
license · from DataCite
- IsSupplementTo 10.1080/15321819.2014.996817 doi.org/10.1080/15321819.2014.996817 ↗
publication · from DataCite
Catalogue records · 4
- DataCite API api.datacite.org/dois/10.6084/m9.figshare.1275093.v2 ↗
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- DataCite API api.datacite.org/dois/10.6084/m9.figshare.1275093 ↗
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- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.1275093.v2 ↗
catalogue entry · from DataCite
- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.1275093 ↗
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Topics
- Stated by source
- Biological sciences · Biological sciences · Health sciences · Health sciences
- From keywords
- Cardiology · Genetics · Infectious diseases · Life Sciences · Life Sciences
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Provenance · 2 source records, 14 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DataCite | 10.6084/m9.figshare.1275093 | 14 d ago | JSON v1 |
| DataCite | 10.6084/m9.figshare.1275093.v2 | 14 d ago | JSON v1 |
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