Data Sheet 1_Coordinated epigenomic reprogramming and chromatin-mediated transcriptional regulation during soybean callus development.zip

However, the epigenetic mechanisms underlying this process remain poorly understood. </p>Methods<p>Here, we performed integrative analyses of time-series ATAC-seq, ChIP-seq (H3K27ac and H3K27me3), and transcriptome datasets to investigate chromatin-mediated transcriptional regulation during soybean callus development. </p>Results<p>Genome-wide profiling identified extensive epigenetic remodeling during early callus induction, including 15,740 differentially accessible regions (DARs) associated with dynamic transcriptional changes.

Distinct chromatin accessibility patterns corresponded to sequential developmental programs, including repression of explant identity, activation of hormone-responsive cell proliferation pathways, and stress-associated responses during later stages. Chromatin accessibility and H3K27ac were strongly associated with transcriptional activation during early induction, whereas de novo open chromatin regions (OCRs) were closely associated with increased expression of key developmental regulators.