<p><i>evm.TU.LG18.404</i> encodes a fish-specific HECT E3 ubiquitin ligase.</p>

The phylogenetic tree was constructed using the neighbor-joining method in MEGA software. The accession numbers for the G2E3 amino acid sequences of each species are as follows: <i>Chanodichthys erythropterus</i> XP_067250078.1; <i>Ctenopharyngodon idella</i> XP_048066262.1; <i>Anabarilius grahami</i> XP_069428.1; <i>Pseudocoregonus laevis</i> XP_067250078.1; <i>A</i>. <i>grahami</i> XP_026099905.1; <i>Megalobrama amblycephala</i> XP_048066262.1; <i>A</i>. <i>grahami</i> ROI69428.1; <i>Pseudorasbora parva</i> XP_067268633.1; <i>Phoxinus phoxinus</i> KAK7135471.1; <i>Labeo rohita</i> KAI2661412.1; <i>Sinocyclocheilus anshuiensis</i> XP_016360734.1; <i>Cyprinus carpio</i> KTF85257.1; <i>Carassius auratus</i> XP_026099905.1; <i>Carassius gibelio</i> XP_052459386.1; <i>Triplophysa tibetana</i> KAA0713717.1; <i>Drosophila pseudoobscura</i> XP_001354945.2; <i>Parasteatoda tepidariorum</i> XP_015912803.2; <i>Lissotriton helveticus</i> XP_078515543.1; <i>Lacerta agilis</i> XP_033000160.1; <i>Gallus gallus</i> NP_001012918.2; <i>Mus musculus</i> NP_001015099.2; <i>Canis lupus familiaris</i> XP_013971289.1; <i>Sus scrofa</i> XP_001927828.4; <i>Homo sapiens</i> NP_060239.2; <i>Macaca mulatta</i> NP_001181581.2.

(B) Comparison of G2E3 protein sequence similarity among fish and other species. Sequence similarity is markedly lower in fish than in other species. (C) Comparison of NHRE3, G2E3, and NEULR3 domains.