Omics · study · 2026
Peptide E from Bifidobacterium adolescentis LA20-k9 fermentation inhibits feline renal cellular senescence via the SMAD7/p-SMAD3/IL6-CXCL8 axis
Listed in NCBI GEO
Aging is an inevitable biological phenomenon that drives progressive functional decline at cellular and tissue levels, with chronic kidney disease (CKD) affecting up to 80% of cats over 15 years of age.
Description
Probiotic-derived metabolites hold anti-aging promise, but their renal protective mechanisms remain largely undefined. In this study, we investigated the anti-senescence effects of fermented brown algae extracts from Bifidobacterium adolescentis LA20-K9 (LA20FM) on feline kidney cells.
Both the LA20FM<3 kDa and LA20FM>30 kDa molecular weight fractions mitigated doxorubicin-induced senescence, as evidenced by reduced SA-β-gal activity and decreased CDKN1A/CDKN2A expression. Peptide E, which was isolated from the LA20FM<3 kDa fraction, was identified as the principal bioactive component. RNA-sequencing revealed that peptide E downregulated senescence-associated genes, including IL6, CXCL8, GDF15, and KRT15, while upregulating the anti-senescence gene SMAD7.
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Mechanistically, peptide E enhanced SMAD7 expression, which in turn suppressed SMAD3 phosphorylation and consequently reduced IL6 and CXCL8 transcription. Crucially, SMAD7 knockdown completely abrogated these protective effects. Collectively, our findings identify peptide E as a novel bioactive peptide that antagonizes renal cellular senescence via the SMAD7/p-SMAD3/IL6-CXCL8 axis, offering a potential therapeutic candidate for age-related kidney dysfunction in felines.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337653 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337653 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1490543 ncbi.nlm.nih.gov/bioproject/PRJNA1490543 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Disease 75%
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE337653 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |