Gene expression analysis of native and invasive populations Centaurea diffusa under control and drought treatments

TAIR 10 accessions (The Arabidopsis Information Resource; arabidopsis.org) and GO term mappings are included where possible. Table S3: GOresults_sigTrt_pc.slsx GO terms for gene with a significant effect of drought treatment. Results of Fisher’s exact tests examining the number of gene associations with GO terms.

Genes of interest were determined in mixed model analysis to have a significant differences between control and drought treatments. Ontology type is indicated for each term; here, biological process only (BP). The analysis was conducted using the Fisher algorithm and parent-child method in topGO, and P-values for this test are reported.

Direction of expression was determined by relative range level averages of expression all first time point samples, before onset of treatment, and is expressed in terms of invasive relative to native samples. Separate topGO analyses of up or down regulated genes were then used to confirm association with GO terms.