Data · dataset · 2026
Identification and bioactivity evaluation of a novel temporin peptide, QUB-1347, from the skin secretion of Rana dybowskii
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32640075.v1
At present, many pathogenic microorganisms have developed serious resistance, so scientists are working to find a new type of treatment drugs to replace traditional antibiotics.
Description
Antimicrobial peptides (AMPs) have a broad spectrum of antibacterial activity and have attracted great interest from scientists because of their potential biological activities against bacteria, fungi, viruses and cancer cells. <br><br>In this study, shotgun cloning was utilized to isolate and identify a cDNA encoding an AMP precursor from the skin secretion of Rana dybowskii.
The mature peptide that was obtained, FIGPIISALASLF-NH2, has 14 amino acid residues. Based on its molecular mass, this peptide was given the name QUB-1347. After sufficient peptides were obtained via SPPS, QUB-1347 was subjected to a number of tests to ascertain its biological activity. <br><br>In the antimicrobial assay, QUB-1347 showed significant antimicrobial activity only against Gram-positive bacteria, but not against Gram-negative bacteria and yeast.
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Staphylococcus aureus (Gram-positive bacterium), Escherichia coli (Gram-negative bacterium) and Candida albicans (yeast), had MICs of 16, >128 and >128 µM respectively. Meanwhile, QUB-1347 showed low cytotoxicity to equine red cells, it demonstrated haemolysis of less than 20% at 64 µM. Moreover, QUB-1347 had antiproliferative activity on H838 cancer cells, and the effect was significantly enhanced when the concentration was higher than 25 µM, and the IC50 was 18.65 µM.<br><br><i>Thesis is embargoed until 31 December 2029</i>.<br>
Links
Where it is published
- DOI doi.org/10.17034/32640075.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Life Sciences · Life Sciences · Life Sciences
- Inferred from text
- Cancer 75%
Provenance · 3 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32640075 | 12 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32640075 | 12 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32640075 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |