Egg transcriptome assembly of Meloidogyne enterolobii (INRA/JKI)

From the raw Illumina reads, adapters and low quality regions (Phred score <30) as well as regions that contained 2 or more consecutive ambiguous nucleotides were cropped using prinseq resulting in 54,424,802 cleaned paired-end reads. The clean reads were assembled using CLC Genomics Workbench 9.0 (qiagenbioinformatics.com/) with automatic bubble size and word size estimation, in simple contig mode, and minimum contig length of 200.