Data Sheet 2_Dose-response-defined physiological framework enables comparative analysis of transcriptional response dynamics across multiple herbicides in Arabidopsis thaliana.zip

However, it remains unclear how plants differentially respond to herbicides with distinct modes of action when applied at herbicide-specific treatment levels corresponding to comparable degrees of growth inhibition. In this study, we integrated physiological and transcriptomic analyses to characterize the responses of Arabidopsis thaliana exposed to seven herbicides (2,4-dichlorophenoxyacetic acid, acifluorfen, atrazine, glyphosate, imazethapyr, mesotrione, and paraquat) representing seven different modes of action.

Dose-response experiments were first conducted to determine herbicide-specific inhibitory concentrations, establishing a framework of physiologically defined treatment levels based on growth inhibition for subsequent RNA sequencing. Comparative transcriptomic analysis under a pairwise design with two points (4 hour and 24 hour) and two dosage levels (half-maximal inhibitory concentration and 99% inhibitory concentration) at each time point revealed herbicide-specific differentially expressed genes.